The total viable count was expressed as mean ± standard deviation. The result thus obtained was evaluated using SPSS version 21 (IBM. The predisinfectant count was compared with postdisinfectant count. The number of colony-forming units (CFUs) of the viable microorganisms on the Petri plates was counted using a digital colony counter. The Petri plates were examined for recording the total viable count following incubation. These Petri plates were then inoculated and incubated at 37☌ for 24 h. Pour-plate technique was employed to uniformly dispense the diluted samples on the Petri plates containing the nutrient agar. Nutrient agar was used as a media to elicit the growth of microorganisms. We established three groups: Group I in which impression material was disinfected with glutaraldehyde (2.2%) by soaking it in solution for 5 min, Group II in which UV radiation was used for disinfection consisting of an outer body and inner body chamber used for the study and alginate impressions were placed in the chamber for 20 min, and Group III in which sterilization with autoclave for 15 min under 20 lbs pressure was done.Īll the impressions were subjected to microbial assessment. The institutional ethical committee approved the study protocol. It consisted of sixty alginate impression materials. The present study was conducted in the department of prosthodontic. The present study was conducted to compare various methods of disinfection of impression materials such as glutaraldehyde, ultraviolet (UV) radiation, and autoclave. Sterilization is a preferred method of cross infection control in the dental clinics. ![]() Unlike disinfection, sterilization is a procedure that guarantees the elimination of all microorganisms. It is proposed that all dental prostheses and prosthodontic items should be cleaned, disinfected, and rinsed before they are handled in the laboratory using an active hospital disinfectant.ĭisinfection is either high-level disinfection which involves bacterial spore inactivity along with other microbial forms, intermediate-level disinfection involves destruction of microorganisms such as tubercle bacilli, and low-level disinfection possesses narrow antimicrobial activity.ĭental impression materials can be disinfected by soaking in chemical disinfectant, autoclave, radiation, etc., The disinfectant solution should show high effectiveness in the reduction of pathogenic microorganisms without interfering with the dimensional stability or ability to reproduce details of the material. Rinsing with water was the easiest and frequently used method. Various methods for disinfection of impression materials are available. It is evident in research that contaminated impressions can cross infect gypsum casts that were poured against them. Cross infection between dental clinics and dental laboratories are common. ![]() Dental professionals dealing with impression material come in contact with saliva, and hence, there are chances to get infected if not handled carefully. Materials are frequently contaminated with oral fluids such as saliva and blood. ![]() The oral cavity is full of fluids such as saliva and blood. Dental impression is frequently taken in patients requiring removable, partial, or complete dentures.
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